Hotspot

rrn5-trnR-ACG

Genome · 119765-120012 · 248 bp

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Pi 0.119

MarkerSeek score 77.70

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: Genome
length_bp: 248
variable_sites: 100
pi: 0.119
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	CCTTGTTCCTACATGACCCG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.0	58.0	1271-1275 bp	1.0	91.8
rrn5-trnR-ACG_p2	TGTCCCCTCCAGTCAAGAAT	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.0	58.0	1111-1115 bp	1.0	91.6
rrn5-trnR-ACG_p3	CAGCTGAGGCATCCTAACAG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.0	58.0	1306-1310 bp	1.0	91.6
rrn5-trnR-ACG_p4	CCTTGTTCCTACATGACCCG	TAGCGCATCAAAGATGCAGT	55.0	45.0	58.0	58.0	1048-1052 bp	1.0	91.6
rrn5-trnR-ACG_p5	TGCCTCTCCAAGGGATGTAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.0	58.0	1149-1151 bp	0.285714	51.3