Hotspot

rpoB-clpP

Genome · 81046-83782 · 2737 bp

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Pi 0.0458

MarkerSeek score 80.80

Primer available yes

Label rpoB-clpP

Feature Metadata

label_name: rpoB-clpP
feature_type: igs
region: Genome
length_bp: 2737
variable_sites: 325
pi: 0.0458
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-clpP_p1	TAAACCCAGGTATTGCCGAC	ATGTCAGCAAAAGAAGCCCA	50.0	45.0	57.6	58.0	2832-2957 bp	1.0	88.7
rpoB-clpP_p2	CAGGTATTGCCGACATTCCT	ATGTCAGCAAAAGAAGCCCA	50.0	45.0	57.7	58.0	2852-2951 bp	0.857143	82.2
rpoB-clpP_p3	GTTCTTCCATCAAGCCCTGA	ATGTCAGCAAAAGAAGCCCA	50.0	45.0	57.5	58.0	2889-2964 bp	0.857143	81.6
rpoB-clpP_p4	AGTTCTTCCATCAAGCCCTG	ATGTCAGCAAAAGAAGCCCA	50.0	45.0	57.5	58.0	2890-2965 bp	0.857143	81.5
rpoB-clpP_p5	CAGGTATTGCCGACATTCCT	TGTCAGCAAAAGAAGCCCAA	50.0	45.0	57.7	58.2	2851-2950 bp	0.857143	80.4