Hotspot

atpF

LSC · 12917-14727 · 1811 bp

Back to Uvaria

Pi 0.0269
MarkerSeek score 66.30
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1811
variable_sites
42
pi
0.0269
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 CTCGAAGGGTTCCCATTAGC AAGGGGACGAGGTGATACAA 55.0 50.0 58.0 58.1 1722-1969 bp 1.0 85.0
atpF_p2 CTCGAAGGGTTCCCATTAGC AGGGGACGAGGTGATACAAA 55.0 50.0 58.0 58.1 1721-1968 bp 1.0 85.0
atpF_p3 GCTCGAAGGGTTCCCATTAG AAGGGGACGAGGTGATACAA 55.0 50.0 58.0 58.1 1723-1970 bp 1.0 85.0
atpF_p4 GCTCGAAGGGTTCCCATTAG AGGGGACGAGGTGATACAAA 55.0 50.0 58.0 58.1 1722-1969 bp 1.0 85.0
atpF_p5 CTCGAAGGGTTCCCATTAGC AAAAACAAGGGAAGGGGACG 55.0 50.0 58.0 58.3 1733-1980 bp 1.0 83.0