Hotspot

ycf2-trnI-CAU

IRa · 179339-179798 · 460 bp

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Pi 0.0118
MarkerSeek score 46.30
Primer available yes
Label ycf2-trnI-CAU

Feature Metadata

label_name
ycf2-trnI-CAU
feature_type
igs
region
IRa
length_bp
460
variable_sites
4
pi
0.0118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.74
markerseek_score
46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnI-CAU_p1 TTGTGCGAAAGTAGGATCCG GTTGGGCGCTTTAACCATTC 50.0 50.0 58.0 58.0 707-764 bp 1.0 85.6
ycf2-trnI-CAU_p2 CGAAAGTAGGATCCGCCAAA GTTGGGCGCTTTAACCATTC 50.0 50.0 58.0 58.0 702-759 bp 1.0 85.5
ycf2-trnI-CAU_p3 GTTGAACCCTGCGAATTGTG GTTGGGCGCTTTAACCATTC 50.0 50.0 57.9 58.0 722-779 bp 1.0 85.2
ycf2-trnI-CAU_p4 TTGTGCGAAAGTAGGATCCG CACTTGACTGTCTACGGCTC 50.0 55.0 58.0 58.0 1286-1343 bp 1.0 84.8
ycf2-trnI-CAU_p5 CGAAAGTAGGATCCGCCAAA CACTTGACTGTCTACGGCTC 50.0 55.0 58.0 58.0 1281-1338 bp 1.0 84.7