Hotspot

ycf1-trnN-GUU

IRa · 155383-156140 · 758 bp

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Pi 0.0536
MarkerSeek score 77.50
Primer available yes
Label ycf1-trnN-GUU

Feature Metadata

label_name
ycf1-trnN-GUU
feature_type
igs
region
IRa
length_bp
758
variable_sites
39
pi
0.0536
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnN-GUU_p1 AAACGTTGACCAATCTGGCT AAGCAAAAATGCGTTCCTCG 45.0 45.0 58.0 57.9 1636-1751 bp 1.0 82.9
ycf1-trnN-GUU_p2 CGTCGAACGCTGAAATTACG AAGCAAAAATGCGTTCCTCG 50.0 45.0 58.0 57.9 1553-1668 bp 1.0 82.9
ycf1-trnN-GUU_p3 CGTCGAACGCTGAAATTACG GGAGAATCGACGAATGCACT 50.0 50.0 58.0 58.1 1495-1610 bp 1.0 82.9
ycf1-trnN-GUU_p4 AAACGTTGACCAATCTGGCT GGAGAATCGACGAATGCACT 45.0 50.0 58.0 58.1 1578-1693 bp 1.0 82.8
ycf1-trnN-GUU_p5 AAACGTTGACCAATCTGGCT TCAAAGCGAGCCCTTTCATT 45.0 45.0 58.0 58.1 1352 bp 0.5 59.1