Hotspot

trnN-GUU-ycf1

IRb · 118270-119027 · 758 bp

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Pi 0.0536
MarkerSeek score 77.50
Primer available yes
Label trnN-GUU-ycf1

Feature Metadata

label_name
trnN-GUU-ycf1
feature_type
igs
region
IRb
length_bp
758
variable_sites
39
pi
0.0536
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ycf1_p1 AAGCAAAAATGCGTTCCTCG AAACGTTGACCAATCTGGCT 45.0 45.0 57.9 58.0 1636-1751 bp 1.0 82.9
trnN-GUU-ycf1_p2 AAGCAAAAATGCGTTCCTCG CGTCGAACGCTGAAATTACG 45.0 50.0 57.9 58.0 1553-1668 bp 1.0 82.9
trnN-GUU-ycf1_p3 GGAGAATCGACGAATGCACT CGTCGAACGCTGAAATTACG 50.0 50.0 58.1 58.0 1495-1610 bp 1.0 82.9
trnN-GUU-ycf1_p4 GGAGAATCGACGAATGCACT AAACGTTGACCAATCTGGCT 50.0 45.0 58.1 58.0 1578-1693 bp 1.0 82.8
trnN-GUU-ycf1_p5 TCAAAGCGAGCCCTTTCATT AAACGTTGACCAATCTGGCT 45.0 45.0 58.1 58.0 1352 bp 0.5 59.1