Hotspot

trnI-CAU-ycf2

IRb · 94612-95104 · 493 bp

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Pi 0.0322
MarkerSeek score 58.80
Primer available yes
Label trnI-CAU-ycf2

Feature Metadata

label_name
trnI-CAU-ycf2
feature_type
igs
region
IRb
length_bp
493
variable_sites
12
pi
0.0322
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.76
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC TTGTGCGAAAGTAGGATCCG 50.0 50.0 58.0 58.0 707-764 bp 1.0 85.6
trnI-CAU-ycf2_p2 GTTGGGCGCTTTAACCATTC CGAAAGTAGGATCCGCCAAA 50.0 50.0 58.0 58.0 702-759 bp 1.0 85.5
trnI-CAU-ycf2_p3 CACTTGACTGTCTACGGCTC TTGTGCGAAAGTAGGATCCG 55.0 50.0 58.0 58.0 1286-1343 bp 1.0 84.8
trnI-CAU-ycf2_p4 CACTTGACTGTCTACGGCTC CGAAAGTAGGATCCGCCAAA 55.0 50.0 58.0 58.0 1281-1338 bp 1.0 84.7
trnI-CAU-ycf2_p5 GTTGGGCGCTTTAACCATTC AATCAAGGTCCTTGCGGATC 50.0 50.0 58.0 58.0 1210-1267 bp 1.0 84.7