Hotspot

trnM-CAU-ycf4

LSC · 57583-58556 · 974 bp

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Pi 0.0165
MarkerSeek score 63.40
Primer available yes
Label trnM-CAU-ycf4

Feature Metadata

label_name
trnM-CAU-ycf4
feature_type
igs
region
LSC
length_bp
974
variable_sites
16
pi
0.0165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-ycf4_p1 TACGGCGAAAGTCATTGGTT ACAAAAAGCTGCGGAATTGG 45.0 45.0 57.8 57.8 1067-1071 bp 1.0 79.1
trnM-CAU-ycf4_p2 ATACGGCGAAAGTCATTGGT ACAAAAAGCTGCGGAATTGG 45.0 45.0 57.6 57.8 1068-1072 bp 1.0 77.3
trnM-CAU-ycf4_p3 TACGGCGAAAGTCATTGGTT TGCGGAATTGGCCTATTTCT 45.0 45.0 57.8 57.6 1058-1062 bp 1.0 76.9
trnM-CAU-ycf4_p4 CCCAGTGGTTAGAGTGTTGC ACAAAAAGCTGCGGAATTGG 55.0 45.0 58.5 57.8 1092-1096 bp 1.0 76.6
trnM-CAU-ycf4_p5 TGTTGCTTTCATACGGCGAA ACAAAAAGCTGCGGAATTGG 45.0 45.0 58.5 57.8 1078-1082 bp 1.0 76.6