Hotspot

trnT-UGU-trnL-UAA

LSC · 50157-51329 · 1173 bp

Back to Uvaria

Pi 0.0154
MarkerSeek score 66.50
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1173
variable_sites
18
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 1234-1244 bp 1.0 79.0
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC CCGTAGCGTCTACCAATTCC 55.0 55.0 58.2 58.2 1265-1275 bp 1.0 78.7
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG ATCCAATCAAGTCCGTAGCG 55.0 50.0 57.9 57.8 1246-1256 bp 1.0 78.4
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 1237-1247 bp 1.0 78.2
trnT-UGU-trnL-UAA_p5 GAGTCGAACCGATGACCATC ATCCAATCAAGTCCGTAGCG 55.0 50.0 58.2 57.8 1277-1287 bp 1.0 78.1