Hotspot

atpF-atpH

LSC · 14728-15107 · 380 bp

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Pi 0.0289
MarkerSeek score 70.80
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
380
variable_sites
11
pi
0.0289
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 TATTCAACCCGAAACTCCCG GTTGTGGCATTAGCGCTTTT 50.0 45.0 57.6 57.9 492-510 bp 1.0 78.1
atpF-atpH_p2 CCAGTAACCCAAGGAAACGA GTTGTGGCATTAGCGCTTTT 50.0 45.0 57.4 57.9 464-482 bp 1.0 76.9
atpF-atpH_p3 GGCCAGTAACCCAAGGAAAC GTTGTGGCATTAGCGCTTTT 55.0 45.0 58.7 57.9 466-484 bp 1.0 75.4
atpF-atpH_p4 TATTCAACCCGAAACTCCCG CCGGAAGCAGAGGGTAAAAT 50.0 50.0 57.6 57.6 570-588 bp 1.0 75.0
atpF-atpH_p5 TATTCAACCCGAAACTCCCG ATTTACGGACTGGTTGTGGC 50.0 50.0 57.6 58.5 504-522 bp 1.0 74.8