Hotspot

trnS-GCU-trnG-UCC

LSC · 9314-10084 · 771 bp

Back to Uvaria

Pi 0.00908
MarkerSeek score 64.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
771
variable_sites
7
pi
0.00908
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA GTTTGTCGGTCGGAATCTGA 45.0 50.0 58.0 57.9 907-908 bp 1.0 79.7
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA AGTTTGTCGGTCGGAATCTG 45.0 50.0 58.0 57.9 908-909 bp 1.0 79.7
trnS-GCU-trnG-UCC_p3 TTTCAAGCGATCAGAAGGGG GTTTGTCGGTCGGAATCTGA 50.0 50.0 57.9 57.9 975-976 bp 1.0 79.1
trnS-GCU-trnG-UCC_p4 TTTCAAGCGATCAGAAGGGG AGTTTGTCGGTCGGAATCTG 50.0 50.0 57.9 57.9 976-977 bp 1.0 79.0
trnS-GCU-trnG-UCC_p5 CGGAGAGAGAGGGATTCGAA GTTTGTCGGTCGGAATCTGA 55.0 50.0 58.3 57.9 955-956 bp 1.0 77.4