Hotspot

ycf1

SSC · 105790-110418 · 4629 bp

Back to Utricularia

Pi 0.156

MarkerSeek score 78.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4629
variable_sites: 2766
pi: 0.156
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGATTCCAGCGTTTCGAAT	TTAGGCAAAATACCCTCGCC	45.0	50.0	58.0	58.0	330 bp	0.04	41.2
ycf1_p2	CACCACTTTCGGATATGGCA	TTAGGCAAAATACCCTCGCC	50.0	50.0	58.0	58.0	418 bp	0.04	40.9
ycf1_p3	TTTCAGTTCTGGGAGCCCTA	TTAGGCAAAATACCCTCGCC	50.0	50.0	58.0	58.0	382 bp	0.04	40.9
ycf1_p4	AAAGGTCGATTCCAGCGTTT	TTAGGCAAAATACCCTCGCC	45.0	50.0	58.1	58.0	335 bp	0.04	40.4
ycf1_p5	CGATGTATCGCAAAGGTCGA	TTAGGCAAAATACCCTCGCC	50.0	50.0	58.2	58.0	346 bp	0.04	39.9