Hotspot

rps16

LSC · 5053-6147 · 1095 bp

Back to Utricularia

Pi 0.103

MarkerSeek score 82.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1095
variable_sites: 487
pi: 0.103
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AACCCTGTTCCGTTATCACG	CTACTGTGTACCGCTGAACC	50.0	55.0	57.9	58.0	1705 bp	0.04	40.8
rps16_p2	AATAGACGCAACCCTGTTCC	CTACTGTGTACCGCTGAACC	50.0	55.0	57.9	58.0	1714 bp	0.04	40.7
rps16_p3	AACCCTGTTCCGTTATCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	2160 bp	0.04	40.3
rps16_p4	AACCCTGTTCCGTTATCACG	GTACCGCTGAACCAATGACT	50.0	50.0	57.9	57.9	1698 bp	0.04	40.1
rps16_p5	AATAGACGCAACCCTGTTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	2169 bp	0.04	40.1