Hotspot

rpoA

LSC · 72272-73339 · 1068 bp

Back to Utricularia

Pi 0.0709

MarkerSeek score 82.70

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1068
variable_sites: 343
pi: 0.0709
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TTGGTTGGGTATTGGTGCAA	GAACTGGTTCGCGTAGGAAT	45.0	50.0	57.9	58.0	1673-1713 bp	0.76	80.0
rpoA_p2	TTTGGTTGGGTATTGGTGCA	GAACTGGTTCGCGTAGGAAT	45.0	50.0	57.9	58.0	1674-1714 bp	0.76	80.0
rpoA_p3	TTTCATGTCCGGTTCCTTGG	GAACTGGTTCGCGTAGGAAT	50.0	50.0	58.1	58.0	2141-2181 bp	0.68	76.2
rpoA_p4	TTCAATGATAGGCGAACCGG	GAACTGGTTCGCGTAGGAAT	50.0	50.0	58.1	58.0	1953 bp	0.04	41.1
rpoA_p5	TTCAATGATAGGCGAACCGG	TTGTCCGAGATGTAACCCCT	50.0	50.0	58.1	58.1	1620 bp	0.04	40.8