Hotspot

petB

LSC · 69267-70656 · 1390 bp

Back to Utricularia

Pi 0.0669

MarkerSeek score 82.70

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1390
variable_sites: 406
pi: 0.0669
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	ACCACCTCGAGTTCCAACTA	CTTATGCACAGCACAACACG	50.0	50.0	58.0	58.0	2441 bp	0.04	41.2
petB_p2	GGTAAAGTAGCTCCCGGTTG	CTTATGCACAGCACAACACG	55.0	50.0	58.0	58.0	2012 bp	0.04	41.1
petB_p3	GTAAAGTAGCTCCCGGTTGG	CTTATGCACAGCACAACACG	55.0	50.0	58.0	58.0	2011 bp	0.04	41.1
petB_p4	AAGGTTGCCCAAAAGCAGTA	CTTATGCACAGCACAACACG	45.0	50.0	57.9	58.0	2303 bp	0.04	40.6
petB_p5	ACCACCTCGAGTTCCAACTA	AGATCTTACGGAGCCCACTT	50.0	50.0	58.0	58.1	2458 bp	0.04	40.5