Hotspot

accD

LSC · 52946-54457 · 1512 bp

Back to Utricularia

Pi 0.065

MarkerSeek score 83.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 533
pi: 0.065
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGTCTTTTTCGTTCCGTGGT	AAAGAAGCCATCGCAATTGC	45.0	45.0	58.0	58.0	2348-2514 bp	0.36	64.4
accD_p2	TGTCTTTTTCGTTCCGTGGT	ACATCTGCATTCATCGACCC	45.0	50.0	58.0	58.0	2603 bp	0.04	41.1
accD_p3	TGTCTTTTTCGTTCCGTGGT	GCATTCATCGACCCTGATGT	45.0	50.0	58.0	58.0	2597 bp	0.04	41.1
accD_p4	TGTCTTTTTCGTTCCGTGGT	TGCATTCATCGACCCTGATG	45.0	50.0	58.0	58.0	2598 bp	0.04	41.0
accD_p5	TCCACAATTAAACCCACGGG	AAAGAAGCCATCGCAATTGC	50.0	45.0	58.1	58.0	2460 bp	0.04	40.9