Hotspot

rps15-ycf1

SSC · 105484-105789 · 306 bp

Back to Utricularia

Pi 0.244

MarkerSeek score 71.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 306
variable_sites: 244
pi: 0.244
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGTTTGCAGCCAAAACTTCA	GGGAATGGTGCTTTGACTCA	45.0	50.0	58.1	58.1	1530 bp	0.04	40.2
rps15-ycf1_p2	GTCAATCCATGCCTCTGTGT	GGGAATGGTGCTTTGACTCA	50.0	50.0	57.9	58.1	1565 bp	0.04	39.9
rps15-ycf1_p3	CGTTTGCAGCCAAAACTTCA	CTTTTTCAATTGCGCGGGAA	45.0	45.0	58.1	58.2	1545 bp	0.04	39.2
rps15-ycf1_p4	GTCAATCCATGCCTCTGTGT	CTTTTTCAATTGCGCGGGAA	50.0	45.0	57.9	58.2	1580 bp	0.04	38.9
rps15-ycf1_p5	TTTCTTCAATCCGTGGCAGT	GGGAATGGTGCTTTGACTCA	45.0	50.0	57.7	58.1	1392 bp	0.04	38.7