Hotspot

rpoB-trnC-GCA

LSC · 25935-27085 · 1151 bp

Back to Utricularia

Pi 0.186

MarkerSeek score 80.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 776
pi: 0.186
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TTTTCTACTAACGCGAGCCC	TTGCAAGCCTTGGTCTTGAT	50.0	45.0	58.0	58.0	1976 bp	0.04	41.6
rpoB-trnC-GCA_p2	ATACTGGATCCCCACCTACG	TTGCAAGCCTTGGTCTTGAT	55.0	45.0	58.0	58.0	2166 bp	0.04	41.5
rpoB-trnC-GCA_p3	CCACCTACGCAAGCAAATTG	TTGCAAGCCTTGGTCTTGAT	50.0	45.0	58.0	58.0	2155 bp	0.04	41.4
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	TTGCAAGCCTTGGTCTTGAT	50.0	45.0	58.0	58.0	1963 bp	0.04	41.4
rpoB-trnC-GCA_p5	TCTACTAACGCGAGCCCATA	TTGCAAGCCTTGGTCTTGAT	50.0	45.0	58.0	58.0	1973 bp	0.04	41.4