Hotspot

rps16-trnQ-UUG

LSC · 6148-6661 · 514 bp

Back to Utricularia

Pi 0.197

MarkerSeek score 74.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 350
pi: 0.197
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGGCAGCAACATACCCTTTT	CTTACAGCTGCTTGCCAAAC	45.0	50.0	58.0	57.9	1382-1891 bp	0.76	81.9
rps16-trnQ-UUG_p2	TGGCAGCAACATACCCTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	829-1294 bp	0.72	80.1
rps16-trnQ-UUG_p3	AGATCCTTGTCCCCGAGAAA	CTTACAGCTGCTTGCCAAAC	50.0	50.0	58.0	57.9	1200-1688 bp	0.64	76.8
rps16-trnQ-UUG_p4	AGCATCCTCATTTCGCGATT	CTTACAGCTGCTTGCCAAAC	45.0	50.0	58.0	57.9	1243 bp	0.04	40.8
rps16-trnQ-UUG_p5	AGCATCCTCATTTCGCGATT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	654 bp	0.04	40.7