Hotspot

trnK-UUU-rps16

LSC · 4170-5052 · 883 bp

Back to Utricularia

Pi 0.207

MarkerSeek score 77.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 602
pi: 0.207
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AACGGCAGGCAATAGTTAGG	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.0	58.0	1876-2065 bp	0.64	77.2
trnK-UUU-rps16_p2	AACGGCAGGCAATAGTTAGG	TCGATCTCGACGAGAAGGAA	50.0	50.0	58.0	58.0	1481-1571 bp	0.44	69.1
trnK-UUU-rps16_p3	TTACACATAGAACGGCAGGC	AAAAGGGTATGTTGCTGCCA	50.0	45.0	58.0	58.0	2019-2076 bp	0.16	58.2
trnK-UUU-rps16_p4	TTACACATAGAACGGCAGGC	TCGATCTCGACGAGAAGGAA	50.0	50.0	58.0	58.0	1535-1561 bp	0.12	56.5
trnK-UUU-rps16_p5	TTACACATAGAACGGCAGGC	TAGTCAACGTGGATCATGCG	50.0	50.0	58.0	58.1	2081 bp	0.04	40.8