Hotspot

matK-trnK-UUU

LSC · 3441-4132 · 692 bp

Back to Utricularia

Pi 0.101

MarkerSeek score 83.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 303
pi: 0.101
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TTGCAATTCCAAGACTCGCT	CGTGATAACGGAACAGGGTT	45.0	50.0	58.1	57.9	1656 bp	0.04	40.1
matK-trnK-UUU_p2	TTGCAATTCCAAGACTCGCT	TCGGGTTGCTAACTCAATGG	45.0	50.0	58.1	57.9	1282 bp	0.04	39.9
matK-trnK-UUU_p3	TTGCAATTCCAAGACTCGCT	GGAACAGGGTTGCGTCTATT	45.0	50.0	58.1	57.9	1647 bp	0.04	39.9
matK-trnK-UUU_p4	TTGCAATTCCAAGACTCGCT	CGGGTTGCTAACTCAATGGT	45.0	50.0	58.1	58.2	1281 bp	0.04	39.3
matK-trnK-UUU_p5	TTGCAATTCCAAGACTCGCT	TGATAACGGAACAGGGTTGC	45.0	50.0	58.1	58.2	1654 bp	0.04	39.3