Hotspot

rps15-ycf1

SSC · 117395-117761 · 367 bp

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Pi 0.0535
MarkerSeek score 73.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
367
variable_sites
71
pi
0.0535
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCCCAACGCGTAACATAAGG CTAAAAAGGGCGGGACACTT 50.0 50.0 58.0 58.1 1447-1463 bp 1.0 88.4
rps15-ycf1_p2 TCCCAACGCGTAACATAAGG AACCCCTTCGTCTGTGTCTA 50.0 50.0 58.0 58.0 1464-1477 bp 0.85 82.9
rps15-ycf1_p3 TCCCAACGCGTAACATAAGG ACCCCTTCGTCTGTGTCTAA 50.0 50.0 58.0 58.0 1463-1476 bp 0.85 82.9
rps15-ycf1_p4 AACACCATGCATTGATGGGT AACCCCTTCGTCTGTGTCTA 45.0 50.0 58.1 58.0 1327-1340 bp 0.8 80.7
rps15-ycf1_p5 AACACCATGCATTGATGGGT ACCCCTTCGTCTGTGTCTAA 45.0 50.0 58.1 58.0 1326-1339 bp 0.8 80.7