Hotspot

psbE-petL

LSC · 60612-61682 · 1071 bp

Back to Urtica

Pi 0.03
MarkerSeek score 75.90
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1071
variable_sites
126
pi
0.03
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC TCAAAGCTGCTAGTAGAAAACCA 50.0 39.1 57.8 58.0 1132-1165 bp 0.95 63.4
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC TCAAAGCTGCTAGTAGAAAACCA 55.0 39.1 58.2 58.0 1127-1160 bp 0.95 63.1
psbE-petL_p3 TCTCCTGTGCTTCCAGACAT TCAAAGCTGCTAGTAGAAAACCA 50.0 39.1 58.4 58.0 1125-1158 bp 0.95 61.9
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC GTCAAAGCTGCTAGTAGAAAACC 50.0 43.5 57.8 57.7 1133-1166 bp 0.95 61.4
psbE-petL_p5 GTTCTCCTGTGCTTCCAGAC GTCAAAGCTGCTAGTAGAAAACC 55.0 43.5 58.2 57.7 1128-1161 bp 0.95 61.1