Hotspot

rbcL-accD

LSC · 53375-54092 · 718 bp

Back to Urtica

Pi 0.0344
MarkerSeek score 72.90
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
718
variable_sites
92
pi
0.0344
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCCGTAGCTAATCGAGTAGC TACTGAAACTCCCGCTTTCG 55.0 50.0 58.0 57.9 1423-1479 bp 0.85 85.0
rbcL-accD_p2 GGCTTCAGGGGGTATTCATG TACTGAAACTCCCGCTTTCG 55.0 50.0 58.0 57.9 1541-1597 bp 0.85 84.8
rbcL-accD_p3 GCCGTAGCTAATCGAGTAGC ACTCGCCCTATGACTATCCC 55.0 55.0 58.0 58.1 1116-1172 bp 0.75 80.7
rbcL-accD_p4 GGCTTCAGGGGGTATTCATG ACTCGCCCTATGACTATCCC 55.0 55.0 58.0 58.1 1234-1290 bp 0.75 80.5
rbcL-accD_p5 GAGGCAGCTAAATGGAGTCC ACTCGCCCTATGACTATCCC 55.0 55.0 58.0 58.1 1026-1076 bp 0.25 56.2