Hotspot

trnC-GCA-petN

LSC · 26167-26952 · 786 bp

Back to Urtica

Pi 0.0386
MarkerSeek score 74.10
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
786
variable_sites
111
pi
0.0386
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 841-865 bp 1.0 88.8
trnC-GCA-petN_p2 GGTGTCGCCTGATAACCAAA AGCCCAAGCGAGACTTACTA 50.0 50.0 58.2 58.2 822-846 bp 1.0 88.1
trnC-GCA-petN_p3 TTAGATCGTTTTGGCGGCAT AGCCCAAGCGAGACTTACTA 45.0 50.0 58.3 58.2 895-919 bp 1.0 87.3
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 902-926 bp 1.0 87.1
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 859-883 bp 1.0 86.6