Hotspot

ycf2

IRa · 149176-156051 · 6876 bp

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Pi 0.0134

MarkerSeek score 45.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6876
variable_sites: 92
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GCTGTAGGAGATTTTGTTTGGG	GTTCTTGCAGAGTGGAACCA	45.5	50.0	57.6	58.0	219 bp	1.0	64.2
ycf2_p2	GCTGTAGGAGATTTTGTTTGGG	TTCGGAGTTCTTGCAGAGTG	45.5	50.0	57.6	57.8	225 bp	1.0	63.1
ycf2_p3	GCTGTAGGAGATTTTGTTTGGG	TCTTGCAGAGTGGAACCATG	45.5	50.0	57.6	57.8	217 bp	1.0	63.0
ycf2_p4	GCTGTAGGAGATTTTGTTTGGG	GAGTGGAACCATGCAGTACC	45.5	55.0	57.6	58.3	210 bp	1.0	62.6
ycf2_p5	GCTGTAGGAGATTTTGTTTGGG	AGTTCTTGCAGAGTGGAACC	45.5	50.0	57.6	57.7	220 bp	1.0	62.4