Hotspot

ycf1

IRb · 112457-113537 · 1081 bp

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Pi 0.0167

MarkerSeek score 54.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1081
variable_sites: 18
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	GGGGGCGTATCTCCTCTTAT	55.0	55.0	58.0	58.1	1505-1585 bp	1.0	81.2
ycf1_p2	CAATTCCCGTTCTCAACCCA	GGGGGCGTATCTCCTCTTAT	50.0	55.0	58.1	58.1	1464-1544 bp	1.0	80.3
ycf1_p3	ATCTTTGATGCACTGCGAGT	GGGGGCGTATCTCCTCTTAT	45.0	55.0	57.9	58.1	1822-1901 bp	1.0	80.2
ycf1_p4	ATTCCCGTTCTCAACCCATG	GGGGGCGTATCTCCTCTTAT	50.0	55.0	57.9	58.1	1462-1542 bp	1.0	80.1
ycf1_p5	GCTTCCTTCGTAACTACCGG	GGGGGCGTATCTCCTCTTAT	55.0	55.0	58.1	58.1	1425 bp	0.5	58.9