Hotspot

rrn23-rrn4.5

IRb · 110559-110656 · 98 bp

Back to Urophysa

Pi 0.0306
MarkerSeek score 70.10
Primer available yes
Label rrn23-rrn4.5

Feature Metadata

label_name
rrn23-rrn4.5
feature_type
igs
region
IRb
length_bp
98
variable_sites
3
pi
0.0306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23-rrn4.5_p1 AAGTAGTAAGCCCACCCCAA TCACTGCACTTCCACTTGAC 50.0 50.0 58.2 58.0 194 bp 1.0 78.9
rrn23-rrn4.5_p2 AAGTAGTAAGCCCACCCCAA TGGTCTGTTAGGATGCCTCA 50.0 50.0 58.2 58.0 224 bp 1.0 78.7
rrn23-rrn4.5_p3 AAGTAGTAAGCCCACCCCAA CTGTTAGGATGCCTCAGCTG 50.0 55.0 58.2 58.0 220 bp 1.0 78.7
rrn23-rrn4.5_p4 AAGTAGTAAGCCCACCCCAA GTCTGTTAGGATGCCTCAGC 50.0 55.0 58.2 58.1 222 bp 1.0 78.7
rrn23-rrn4.5_p5 TAGTAAGCCCACCCCAAGAT TCACTGCACTTCCACTTGAC 50.0 50.0 57.7 58.0 191 bp 1.0 78.6