Hotspot

ycf4-cemA

LSC · 63893-64689 · 797 bp

Back to Urophysa

Pi 0.0154

MarkerSeek score 70.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 12
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CGTTCCCTTGACTCGTACTG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.0	58.1	963-973 bp	1.0	81.9
ycf4-cemA_p2	TGCGCGTACCAATTGAAGTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.9	58.1	886-896 bp	1.0	81.6
ycf4-cemA_p3	ACAAAAAGCTGCGGAATTGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	915-925 bp	1.0	80.9
ycf4-cemA_p4	CGTTCCCTTGACTCGTACTG	AAGGGGTTTTAGCGCTTTCT	55.0	45.0	58.0	57.7	920-930 bp	1.0	80.3
ycf4-cemA_p5	CGTTCCCTTGACTCGTACTG	AGGGGTTTTAGCGCTTTCTT	55.0	45.0	58.0	57.7	919-929 bp	1.0	80.3