Hotspot

rps4-trnT-UGU

LSC · 48318-48654 · 337 bp

Back to Urophysa

Pi 0.00898

MarkerSeek score 49.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 337
variable_sites: 3
pi: 0.00898
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAGTCCCGGTAAAACACCCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	462-463 bp	1.0	77.8
rps4-trnT-UGU_p2	TAGTCCCGGTAAAACACCCA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	430-431 bp	1.0	77.0
rps4-trnT-UGU_p3	TAGTCCCGGTAAAACACCCA	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.3	57.5	460-461 bp	1.0	74.7
rps4-trnT-UGU_p4	TAGTCCCGGTAAAACACCCA	TCAGAGGTTAGAGCATCGCA	50.0	50.0	58.3	58.5	461-462 bp	1.0	74.7
rps4-trnT-UGU_p5	TAGTCCCGGTAAAACACCCA	AAGTCCTATGCACAGCACAA	50.0	45.0	58.3	57.4	506-507 bp	1.0	74.2