Hotspot

trnR-UCU-atpA

LSC · 10306-10441 · 136 bp

Back to Urophysa

Pi 0.0242

MarkerSeek score 69.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 3
pi: 0.0242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAATGAAGGGCGTCCATTGT	GAACAGACGGAGCGGTTTAT	45.0	50.0	58.1	58.0	244-256 bp	1.0	84.1
trnR-UCU-atpA_p2	AGGGCGTCCATTGTCTAATG	GAACAGACGGAGCGGTTTAT	50.0	50.0	57.7	58.0	238-250 bp	1.0	82.2
trnR-UCU-atpA_p3	AAATGAAGGGCGTCCATTGT	CGAAGAAGCGGAAGTCCTTT	45.0	50.0	58.1	58.2	281-293 bp	1.0	81.9
trnR-UCU-atpA_p4	AAATGAAGGGCGTCCATTGT	CGACCAAGACATTCACCGAA	45.0	50.0	58.1	58.2	297-309 bp	1.0	81.7
trnR-UCU-atpA_p5	AAATGAAGGGCGTCCATTGT	TTCGACCAAGACATTCACCG	45.0	50.0	58.1	58.2	299-311 bp	1.0	81.6