Hotspot

rpl32-trnL-UAG

SSC · 106943-107459 · 517 bp

Back to Urochloa

Pi 0.0245

MarkerSeek score 69.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 517
variable_sites: 24
pi: 0.0245
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGACCATTTTCTAGCGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.5	57.9	634-639 bp	1.0	79.4
rpl32-trnL-UAG_p2	AGACCATTTTCTAGCGGCAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.5	57.9	633-638 bp	1.0	79.4
rpl32-trnL-UAG_p3	GACCATTTTCTAGCGGCAAC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.5	57.9	632-637 bp	1.0	78.9
rpl32-trnL-UAG_p4	AAGACCATTTTCTAGCGGCA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.5	57.8	627-632 bp	1.0	78.9
rpl32-trnL-UAG_p5	AGACCATTTTCTAGCGGCAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.5	57.8	626-631 bp	1.0	78.9