Hotspot

psbH-petB

LSC · 71815-72644 · 830 bp

Back to Urochloa

Pi 0.0205

MarkerSeek score 64.60

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 31
pi: 0.0205
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TCTTCTGTTTTACTAGACGGA	CCCCTCCGAGAACCGTATAT	38.1	55.0	53.7	58.1	895-900 bp	1.0	43.9
psbH-petB_p2	TCTTCTGTTTTACTAGACGGAA	CCCCTCCGAGAACCGTATAT	36.4	55.0	54.6	58.1	895-900 bp	1.0	43.9
psbH-petB_p3	TTCTTCTGTTTTACTAGACGGA	CCCCTCCGAGAACCGTATAT	36.4	55.0	54.6	58.1	896-901 bp	1.0	43.9
psbH-petB_p4	TTCTTCTGTTTTACTAGACGGAA	CCCCTCCGAGAACCGTATAT	34.8	55.0	55.3	58.1	896-901 bp	1.0	43.9
psbH-petB_p5	TCTTCTGTTTTACTAGACGGA	AGGTAATTAGCCCGACTCCC	38.1	55.0	53.7	58.6	913-918 bp	1.0	43.8