Hotspot

psbE-petL

LSC · 62862-64180 · 1319 bp

Back to Urochloa

Pi 0.0173

MarkerSeek score 67.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1319
variable_sites: 44
pi: 0.0173
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCA	45.0	45.0	58.0	57.3	1477-1491 bp	1.0	77.2
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.0	57.3	1476-1490 bp	1.0	77.2
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1466-1480 bp	1.0	76.9
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1465-1479 bp	1.0	76.9
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.8	57.3	1388-1402 bp	1.0	76.2