Hotspot

trnG-UCC-trnfM-CAU

LSC · 12142-12477 · 336 bp

Back to Urochloa

Pi 0.0342

MarkerSeek score 67.30

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 336
variable_sites: 18
pi: 0.0342
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	GGCGTAGTTCAATGTAGCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	396-496 bp	1.0	84.7
trnG-UCC-trnfM-CAU_p2	GGCGTAGTTCAATGTAGCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	411-511 bp	1.0	84.2
trnG-UCC-trnfM-CAU_p3	GGCGTAGTTCAATGTAGCGA	GAGCGGAGTAGAGCAGTTTG	50.0	55.0	58.1	58.4	424-524 bp	1.0	83.4
trnG-UCC-trnfM-CAU_p4	GGCGTAGTTCAATGTAGCGA	TAGAGCGGAGTAGAGCAGTT	50.0	50.0	58.1	57.6	426-526 bp	1.0	83.0
trnG-UCC-trnfM-CAU_p5	GGCGTAGTTCAATGTAGCGA	AGAGCGGAGTAGAGCAGTTT	50.0	50.0	58.1	58.4	425-525 bp	1.0	82.8