Hotspot

psbK-psbI

LSC · 6904-7227 · 324 bp

Back to Urochloa

Pi 0.0242

MarkerSeek score 66.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 15
pi: 0.0242
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	TCCAAAGATAAAGAGGGAAACA	50.0	36.4	58.0	55.0	455-467 bp	1.0	43.3
psbK-psbI_p2	AGCCGCTGTAAGTTTTCGAT	TCCAAAGATAAAGAGGGAAACA	45.0	36.4	57.9	55.0	453-465 bp	1.0	43.3
psbK-psbI_p3	AAGCCGCTGTAAGTTTTCGA	TCCAAAGATAAAGAGGGAAACA	45.0	36.4	58.1	55.0	454-466 bp	1.0	43.3
psbK-psbI_p4	CGTGGATGTTATGCCTGTCA	TCCAAAGATAAAGAGGGAAACA	50.0	36.4	58.0	55.0	510-522 bp	1.0	43.0
psbK-psbI_p5	ACCCGAAGCTTATGCCATTT	TCCAAAGATAAAGAGGGAAACA	45.0	36.4	57.9	55.0	540-552 bp	1.0	42.8