Hotspot

ycf1

SSC · 123420-128891 · 5472 bp

Back to Urera

Pi 0.0257
MarkerSeek score 58.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5472
variable_sites
353
pi
0.0257
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AAGGGTCGTTTCGTTCCAAA CCCTCGATGGTCACACAAAT 45.0 50.0 58.0 57.9 332-341 bp 1.0 88.9
ycf1_p2 AGGGTCGTTTCGTTCCAAAA CCCTCGATGGTCACACAAAT 45.0 50.0 58.0 57.9 331-340 bp 1.0 88.9
ycf1_p3 AAGGGTCGTTTCGTTCCAAA TTCTTAAATGGTGCCTCCCG 45.0 50.0 58.0 57.9 682-748 bp 1.0 86.1
ycf1_p4 AGGGTCGTTTCGTTCCAAAA TTCTTAAATGGTGCCTCCCG 45.0 50.0 58.0 57.9 681-747 bp 1.0 86.1
ycf1_p5 CGTCGAAAATCCGGTTGTTG CCCTCGATGGTCACACAAAT 50.0 50.0 58.1 57.9 225-234 bp 0.333333 60.7