Hotspot

ccsA-ndhD

SSC · 114804-115083 · 280 bp

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Pi 0.043
MarkerSeek score 64.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
280
variable_sites
34
pi
0.043
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATGCAGCTCTTTTATGCGGA CCGCGGGAATTGTTTCTTTC 45.0 50.0 58.0 58.0 926-939 bp 1.0 85.4
ccsA-ndhD_p2 ATGCAGCTCTTTTATGCGGA TAAAAATGGGCGCGTATGGA 45.0 45.0 58.0 58.0 1540-1553 bp 1.0 83.9
ccsA-ndhD_p3 GCCTCGACGGGTTTTCTTAT CCGCGGGAATTGTTTCTTTC 50.0 50.0 58.0 58.0 511 bp 0.166667 46.3
ccsA-ndhD_p4 GCCTCGACGGGTTTTCTTAT TAAAAATGGGCGCGTATGGA 50.0 45.0 58.0 58.0 1125 bp 0.166667 46.2
ccsA-ndhD_p5 GCCTCGACGGGTTTTCTTAT TGGGTGGAATGGCTATCAGA 50.0 50.0 58.0 58.1 795 bp 0.166667 45.6