Hotspot

ndhF-rpl32

SSC · 111983-112644 · 662 bp

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Pi 0.0349
MarkerSeek score 69.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
662
variable_sites
58
pi
0.0349
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCCTGATCGTGAGACATGT AAAAGCTTTCAATGCTGCCC 50.0 45.0 58.1 57.8 1118-1476 bp 0.833333 75.9
ndhF-rpl32_p2 CACATACGGCGAAGATCCTT AAAAGCTTTCAATGCTGCCC 50.0 45.0 57.8 57.8 877-1235 bp 0.833333 75.6
ndhF-rpl32_p3 ACCAAAATCTCCAACGCGAT AAAAGCTTTCAATGCTGCCC 45.0 45.0 58.2 57.8 1322-1680 bp 0.833333 75.1
ndhF-rpl32_p4 CTCCAACGCGATTAGTCACA AAAAGCTTTCAATGCTGCCC 50.0 45.0 58.0 57.8 1314 bp 0.166667 45.2
ndhF-rpl32_p5 TCTCCAACGCGATTAGTCAC AAAAGCTTTCAATGCTGCCC 50.0 45.0 57.7 57.8 1315 bp 0.166667 43.1