Hotspot

rpl22-rps19

LSC · 84014-84158 · 145 bp

Back to Urera

Pi 0.0629
MarkerSeek score 64.10
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
145
variable_sites
17
pi
0.0629
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCTCCCTTTGCGCCTAAATC GGGCCCCAATTGGTAGAAAA 50.0 50.0 58.0 58.1 934-943 bp 1.0 84.0
rpl22-rps19_p2 GCATAGGAACGTCCACGAAT GGGCCCCAATTGGTAGAAAA 50.0 50.0 58.1 58.1 679-688 bp 1.0 84.0
rpl22-rps19_p3 CTCCCTTTGCGCCTAAATCT GGGCCCCAATTGGTAGAAAA 50.0 50.0 58.0 58.1 933-942 bp 1.0 83.9
rpl22-rps19_p4 TCTCCCTTTGCGCCTAAATC AAAAGTTTGGGTAGAGCCGG 50.0 50.0 58.0 58.1 1040-1049 bp 1.0 83.2
rpl22-rps19_p5 CTCCCTTTGCGCCTAAATCT AAAAGTTTGGGTAGAGCCGG 50.0 50.0 58.0 58.1 1039-1048 bp 1.0 83.1