Hotspot

rpl36-rps8

LSC · 79665-80214 · 550 bp

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Pi 0.0301
MarkerSeek score 67.50
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
550
variable_sites
38
pi
0.0301
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.90
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 AATTTTACGGACGGAGGCTC AGGGAGGCTCGACTAGAAAG 50.0 55.0 58.0 58.2 580-634 bp 1.0 87.1
rpl36-rps8_p2 AATTTTACGGACGGAGGCTC GGGAGGCTCGACTAGAAAGA 50.0 55.0 58.0 58.2 579-633 bp 1.0 87.1
rpl36-rps8_p3 AATTTTACGGACGGAGGCTC TAATGACAGACAGGGAGGCT 50.0 50.0 58.0 57.7 591-645 bp 1.0 86.9
rpl36-rps8_p4 AATTTTACGGACGGAGGCTC ATCCCGAGAATTTTAGCCGG 50.0 50.0 58.0 57.7 646-700 bp 1.0 86.4
rpl36-rps8_p5 AATTTTACGGACGGAGGCTC CCGAGAATTTTAGCCGGGAT 50.0 50.0 58.0 57.7 643-697 bp 1.0 86.4