Hotspot

rpl33-rps18

LSC · 67788-68009 · 222 bp

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Pi 0.0309
MarkerSeek score 64.00
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
222
variable_sites
16
pi
0.0309
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGATTCACGGGGAGATAAAGA AGAGCTGCTTGGATTTAGCC 47.6 50.0 57.3 58.0 270-299 bp 1.0 70.8
rpl33-rps18_p2 CGATTCACGGGGAGATAAAGA GAGCTGCTTGGATTTAGCCA 47.6 50.0 57.3 58.2 269-298 bp 1.0 69.2
rpl33-rps18_p3 CGATTCACGGGGAGATAAAGAA AGAGCTGCTTGGATTTAGCC 45.5 50.0 57.9 58.0 270-299 bp 1.0 68.1
rpl33-rps18_p4 CGATTCACGGGGAGATAAAGA AGCTGCTTGGATTTAGCCAT 47.6 45.0 57.3 57.6 268-297 bp 1.0 67.6
rpl33-rps18_p5 CGATTCACGGGGAGATAAAGAA GAGCTGCTTGGATTTAGCCA 45.5 50.0 57.9 58.2 269-298 bp 1.0 66.6