Hotspot

ycf4-cemA

LSC · 60337-61136 · 800 bp

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Pi 0.0271
MarkerSeek score 64.60
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
800
variable_sites
51
pi
0.0271
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGTGTACCAATTGAAGTT GAGCGATCCACCAAGGTAAA 40.0 50.0 55.9 57.6 838-899 bp 1.0 68.7
ycf4-cemA_p2 TTGCGTGTACCAATTGAAGT GAGCGATCCACCAAGGTAAA 40.0 50.0 55.9 57.6 839-900 bp 1.0 68.7
ycf4-cemA_p3 TGCGTGTACCAATTGAAGTT AGAGCGATCCACCAAGGTAA 40.0 50.0 55.9 58.4 839-900 bp 1.0 68.4
ycf4-cemA_p4 TTGCGTGTACCAATTGAAGT AGAGCGATCCACCAAGGTAA 40.0 50.0 55.9 58.4 840-901 bp 1.0 68.4
ycf4-cemA_p5 TGCGTGTACCAATTGAAGTT ATGAGAGAGCGATCCACCAA 40.0 50.0 55.9 58.5 844-905 bp 1.0 67.7