Hotspot

accD-psaI

LSC · 58675-59241 · 567 bp

Back to Urera

Pi 0.0326
MarkerSeek score 68.80
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
567
variable_sites
50
pi
0.0326
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGTATGGGATCCGTAGTGGG TCGGGGGAAACTACCATTCT 55.0 50.0 58.0 58.0 1272-1338 bp 1.0 86.9
accD-psaI_p2 AACTTTTGCTTGATCCGGGT TCGGGGGAAACTACCATTCT 45.0 50.0 58.0 58.0 1487-1553 bp 1.0 86.3
accD-psaI_p3 AACTTTTGCTTGATCCGGGT TTGCCGGAAATACTAAGCCC 45.0 50.0 58.0 58.0 1338-1390 bp 1.0 85.8
accD-psaI_p4 AGTATGGGATCCGTAGTGGG AAATAGCGGGTAAGGTGCTG 55.0 50.0 58.0 58.0 1090-1128 bp 0.833333 79.2
accD-psaI_p5 AACTTTTGCTTGATCCGGGT AAATAGCGGGTAAGGTGCTG 45.0 50.0 58.0 58.0 1305-1343 bp 0.833333 78.6