Hotspot

trnG-GCC-trnfM-CAU

LSC · 36473-36634 · 162 bp

Back to Urera

Pi 0.0417
MarkerSeek score 64.40
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
162
variable_sites
18
pi
0.0417
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 228-232 bp 1.0 86.5
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 229-233 bp 1.0 86.5
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 264-268 bp 1.0 85.6
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 265-269 bp 1.0 85.6
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC GCAGTTTGGTAGCTTGCAAG 50.0 50.0 58.1 57.9 257-261 bp 1.0 85.5