Hotspot

trnG-UCC-trnR-UCU

LSC · 9698-9967 · 270 bp

Back to Urera

Pi 0.0608
MarkerSeek score 68.20
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
270
variable_sites
37
pi
0.0608
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG TGTCCTATCCATTAGACAATG 50.0 38.1 58.0 52.4 320-340 bp 1.0 52.0
trnG-UCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG TGTCCTATCCATTAGACAATG 55.0 38.1 58.1 52.4 323-343 bp 1.0 52.0
trnG-UCC-trnR-UCU_p3 CCCTAGCCTTCCAAGCTAAC TGTCCTATCCATTAGACAATG 55.0 38.1 57.7 52.4 324-344 bp 1.0 52.0
trnG-UCC-trnR-UCU_p4 CTAACGATGCGGGTTCGATT TGTCCTATCCATTAGACAATG 50.0 38.1 58.4 52.4 309-329 bp 1.0 52.0
trnG-UCC-trnR-UCU_p5 TAACGATGCGGGTTCGATTC TGTCCTATCCATTAGACAATG 50.0 38.1 58.4 52.4 308-328 bp 1.0 52.0