Hotspot

rps16-trnQ-UUG

LSC · 6126-6701 · 576 bp

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Pi 0.0702
MarkerSeek score 71.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
576
variable_sites
82
pi
0.0702
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.90
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 649-702 bp 1.0 90.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA AGCAGCTTGCCAAACAAAAG 45.0 45.0 58.1 58.1 1220-1272 bp 1.0 88.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.1 57.9 1225-1277 bp 1.0 88.9
rps16-trnQ-UUG_p4 ACCCTAGATTCTTGCCTCCA AGCAGCTTGCCAAACAAAAG 50.0 45.0 57.7 58.1 1420-1478 bp 1.0 87.7
rps16-trnQ-UUG_p5 ACCCTAGATTCTTGCCTCCA CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.7 57.9 1425-1483 bp 1.0 87.7