Hotspot

trnK-UUU-rps16

LSC · 4254-4938 · 685 bp

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Pi 0.0457
MarkerSeek score 65.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
685
variable_sites
67
pi
0.0457
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.75
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGACATGCTGCTTTTTCCAG ATTTCAAAGAAGGCGGGTGT 50.0 45.0 58.0 58.0 737-910 bp 1.0 91.0
trnK-UUU-rps16_p2 CGACATGCTGCTTTTTCCAG GAGCCGTACGAGGTGAAAAT 50.0 50.0 58.0 58.0 968-1141 bp 1.0 89.6
trnK-UUU-rps16_p3 GGCCCGATTCCCATAACAAT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 58.0 58.0 956 bp 0.166667 46.6
trnK-UUU-rps16_p4 GGCCCGATTCCCATAACAAT GAGCCGTACGAGGTGAAAAT 50.0 50.0 58.0 58.0 1188 bp 0.166667 46.6
trnK-UUU-rps16_p5 GGCCCGATTCCCATAACAAT CGTTGCAATTGATGTTCGCT 50.0 45.0 58.0 58.0 1107 bp 0.166667 46.4