Hotspot

matK-trnK-UUU

LSC · 3418-4216 · 799 bp

Back to Urera

Pi 0.0221
MarkerSeek score 64.90
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
799
variable_sites
44
pi
0.0221
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 GGGCAAAGGGTATTTCCACA TCAACCGGCGTTTTTCAGTA 50.0 45.0 58.1 58.0 1652-1670 bp 0.333333 58.3
matK-trnK-UUU_p2 TTTACCCAATAGCGAAGGGC TCAACCGGCGTTTTTCAGTA 50.0 45.0 58.0 58.0 1833-1851 bp 0.333333 58.1
matK-trnK-UUU_p3 TTTACCCAATAGCGAAGGGC CGGAAAGAAGGTGCGGATAA 50.0 50.0 58.0 58.0 1997 bp 0.166667 46.1
matK-trnK-UUU_p4 GGGCAAAGGGTATTTCCACA CGGAAAGAAGGTGCGGATAA 50.0 50.0 58.1 58.0 1816 bp 0.166667 46.0
matK-trnK-UUU_p5 GCTCTGCCCGTTAAAGAAGA CGGAAAGAAGGTGCGGATAA 50.0 50.0 57.9 58.0 1847 bp 0.166667 45.8