Hotspot

psaJ

LSC · 65228-65362 · 135 bp

Pi 0.00794

MarkerSeek score 61.40

Primer available yes

Label psaJ

Feature Metadata

label_name: psaJ
feature_type: gene
region: LSC
length_bp: 135
variable_sites: 1
pi: 0.00794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ_p1	CGAACTGAACTAAGAGCGCT	TGGAAGGGTAACACACAAGC	50.0	50.0	58.0	58.0	1389-1411 bp	1.0	81.4
psaJ_p2	CGAACTGAACTAAGAGCGCT	TTTACCCTTGGCCATGAACC	50.0	50.0	58.0	58.1	1171-1193 bp	1.0	81.4
psaJ_p3	ACGTTCTGTACATCACGCTC	TGGAAGGGTAACACACAAGC	50.0	50.0	58.0	58.0	1457-1479 bp	1.0	81.3
psaJ_p4	CGAACTGAACTAAGAGCGCT	CTTGGCCATGAACCTCCTTT	50.0	50.0	58.0	58.1	1165-1187 bp	1.0	81.3
psaJ_p5	GCGTTCTACCGAACTGAACT	TGGAAGGGTAACACACAAGC	50.0	50.0	57.9	58.0	1398-1420 bp	1.0	81.0